Virus-Induced Proteins in Pseudorabies-Infected Cells I. Acid-Extractable Proteins of the Nucleus
نویسندگان
چکیده
منابع مشابه
Phosphorylation of ribosomal proteins in hamster fibroblasts infected with pseudorabies virus or herpes simplex virus.
In BHK cells infected with pseudorabies virus, there was a substantial increase in the phosphorylation of ribosomal protein S6. This increase occurred between 2 and 4 h after infection and persisted at least until 9 h. We estimated that in mock-infected cells S6 contained, on an average, one phosphate group per protein chain, whereas in infected cells this rose to between four and five phosphat...
متن کاملVirus-specific proteins synthesized in encephalomyocarditis virus-infected HeLa cells.
The in vivo synthesis of encephalomyocarditis-specific proteins was studied by labeling the viral proteins with radioactive amino acids under conditions where host-protein synthesis was almost completely inhibited. To assure recovery of all proteins, intact cells were lysed in hot 1% sodium dodecyl sulfate. These lysates were analyzed by quantitative high-resolution electrophoresis on sodium do...
متن کاملI. ANTIGENIC RELATIONSHIP OF VIRuS-STRuCTURAL PROTEINS TO VIRUS-SPECIFIC SOLUBLE ANTIGENS FROM INFECTED CELLS*
Infection of mamm~.lian cells with agents of the adenovirus group elicits production of at least three major soluble antigens. These proteins have been separated on the basis of their immunological, biological, and physiochemical properties into: (a) a group-specific antigen common to all adenovirus types; (b) a type-specific antigen unique for each type; and (c) a toxin-like material which als...
متن کاملAnalysis of proteins synthesized in respiratory syncytial virus-infected cells.
The spectrum of respiratory syncytial virus-encoded proteins was examined in infected cell extracts by standard polyacrylamide gel electrophoresis and by two-dimensional gel analysis. Polyacrylamide gel electrophoresis analysis of a variety of respiratory syncytial virus-infected, actinomycin D-treated cell lines revealed the presence of as many as nine virus-encoded proteins. Seven of these ni...
متن کاملQuantitative whole-cell proteome analysis of pseudorabies virus-infected cells.
A quantitative proteome study using the stable isotope labeling with amino acids in cell culture technique was performed on bovine kidney cells after infection with the alphaherpesvirus pseudorabies virus (PrV), the etiological agent of Aujeszky's disease. To enhance yields of proteins to be identified, raw extracts were fractionated by affinity solid-phase extraction with a combination of a ci...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Virology
سال: 1973
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.11.6.815-822.1973